Microbiology

ISOLATION OF PURE CULTURES A pure floriculture theoretically contains a single bacterial species. There are a number of procedures available for the isolation of pure cultures from mixed populations. A pure culture may be keep apartd by the use of special media with specific chemical or swagger agents that allow the enrichment or selection of one organism all over another. The differential and selective procedures will be utilized after in this course. Simpler methods for isolation of a pure culture intromit: (i) spread plating on solid nutrient agar-agar culture medium with a drinking glass spreader and (ii) runnel plating with a loop. The purpose of spread plating and legal community plating is to isolate individual bacterial cells (colony-forming units) on a solid food medium. two procedures (spread plating and streak plating) charter understanding of the aseptic technique. antisepsis can be delimit as the absence of morbific microorganisms. However, the term is usually employ to any technique knowing to keep unwanted microorganisms from contaminate sterile materials. FIRST consequence Material: 1. 2. 3. 4. 5. Seven 9-ml dilution tubes of sterile saline Seven nutrient agar plates 1.0 ml and 0.1 ml pipets Glass spreader aka field hockey stick 95% ethyl alcoholic drink in glass beaker (WARNING: forbid alcohol away from flame!!) 6.

Mixed nightlong parentage culture of Staphylococcus aureus and Serratia marcescens 27 Procedure: (work in pairs) A. Spread Plate Technique In this technique, the number of bacteria per unit volume of sample is reduced by appendage di lution before the sample is spread on the su! rface of an agar plate. 1. Prepare serial dilutions of the broth culture as shown below. Be sure to mix the nutrient broth tubes before to one after another one serial transfer. Transfer 0.1 ml of the final lead dilutions (10-5, 10-6, 10-7) to each of three nutrient agar plates, and label the plates. 2. site the beaker of alcohol containing the glass spreader away from the flame. Remove the spreader and genuinely carefully pass it over the flame...If you want to startle a full essay, order it on our website: BestEssayCheap.com

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